The Spanish conquest of Yucatán was the campaign undertaken by the Spanish conquistadores against the Late Postclassic Maya states and polities in the Yucatán Peninsula, a vast limestone plain covering south-eastern Mexico, northern Guatemala, and all of Belize. The Spanish conquest of the Yucatán Peninsula was hindered by its politically fragmented state. The Spanish engaged in a strategy of concentrating native populations in newly founded colonial towns. Native resistance to the new nucleated settlements took the form of the flight into inaccessible regions such as the forest or joining neighbouring Maya groups that had not yet submitted to the Spanish. Among the Maya, ambush was a favoured tactic. Spanish weaponry included broadswords, rapiers, lances, pikes, halberds, crossbows, matchlocks, and light artillery. Maya warriors fought with flint-tipped spears, bows and arrows and stones, and wore padded cotton armour to protect themselves. The Spanish introduced a number of Old World diseases previously unknown in the Americas, initiating devastating plagues that swept through the native populations.

The polities of Petén in the south remained independent and received many refugees fleeing from Spanish jurisdiction. In 1618 and in 1619 two unsuccessful Franciscan missions attempted the peaceful conversion of the still pagan Itza. In 1622 the Itza slaughtered two Spanish parties trying to reach their capital Nojpetén. These events ended all Spanish attempts to contact the Itza until 1695. Over the course of 1695 and 1696 a number of Spanish expeditions attempted to reach Nojpetén from the mutually independent Spanish colonies in Yucatán and Guatemala. In early 1695 the Spanish began to build a road from Campeche south towards Petén and activity intensified, sometimes with significant losses on the part of the Spanish. Martín de Urzúa y Arizmendi, governor of Yucatán, launched an assault upon Nojpetén in March 1697; the city fell after a brief battle. With the defeat of the Itza, the last independent and unconquered native kingdom in the Americas fell to the Spanish.