The Nagasaki Naval Training Center was a naval training institute, between 1855 when it was established by the government of the Tokugawa shogunate, until 1859, when it was transferred to Tsukiji in Edo. During the Bakumatsu period, the Japanese government faced increasing incursions by ships from the Western world, intent on ending the country's two centuries of isolationist foreign policy. These efforts cumulated in the landing of United States commodore Matthew Perry in 1854, resulting in the Treaty of Kanagawa and the opening of Japan to foreign trade. The Tokugawa government decided to order modern steam warships and to build a naval training center as part of its modernization efforts to meet the perceived military threat posed by the more advanced Western navies.

Officers of the Royal Netherlands Navy were in charge of education. The curriculum was weighed towards navigation and Western science. The training institute was also equipped with Japan's first steamship, Kankō Maru given by the King of the Netherlands in 1855. It was later joined by the Kanrin Maru and the Chōyō.

The decision to terminate the School was made for political reasons, arising from the Japanese side as well as from the Dutch side. While the Netherlands feared that the other Western powers would suspect that they were helping the Japanese accumulate naval power to repulse Westerners, the Shogunate became reluctant to give samurai from traditionally anti-Tokugawa domains opportunities to learn modern naval technology. Although the Nagasaki Naval Training Center was short-lived, it had considerable direct and indirect influence on future Japanese society. The Nagasaki Naval Training Center educated many naval officers and engineers who would later become not only founders of the Imperial Japanese Navy but also promoters of Japan's shipbuilding and other industries.