The battle opened at dawn (about 5:30 a.m.) on September 17 with an attack down the Hagerstown Turnpike by the Union I Corps under Joseph Hooker. Hooker's objective was the plateau on which sat the Dunker Church, a modest whitewashed building belonging to a local sect of German Baptists. Hooker had approximately 8,600 men, little more than the 7,700 defenders under Stonewall Jackson, and this slight disparity was more than offset by the Confederates' strong defensive positions.^[12] Abner Doubleday's division moved on Hooker's right, James Ricketts's moved on the left into the East Woods, and George Meade's Pennsylvania Reserves division deployed in the center and slightly to the rear. Jackson's defense consisted of the divisions under Alexander Lawton and John R. Jones in line from the West Woods, across the Turnpike, and along the southern end of Miller's Cornfield. Four brigades were held in reserve inside the West Woods.^[13]

As the first Union men emerged from the North Woods and into the Cornfield, an artillery duel erupted. Confederate fire was from the horse artillery batteries under Jeb Stuart to the west and four batteries under Col. Stephen D. Lee on the high ground across the pike from the Dunker Church to the south. Union return fire was from nine batteries on the ridge behind the North Woods and twenty 20-pounder Parrott rifles, 2 miles (3 km) east of Antietam Creek. The conflagration caused heavy casualties on both sides and was described by Col. Lee as "artillery Hell."^[14]

Seeing the glint of Confederate bayonets concealed in the Cornfield, Hooker halted his infantry and brought up four batteries of artillery, which fired shell and canister over the heads of the Federal infantry into the field. A battle began, with considerable melee action with rifle butts and bayonets due to short visibility in the corn. Officers rode about cursing and yelling orders no one could hear in the noise. Rifles became hot and fouled from too much firing; the air was filled with a hail of bullets and shells.

Meade's 1st Brigade of Pennsylvanians, under Brig. Gen. Truman Seymour, began advancing through the East Woods and exchanged fire with Col. James Walker's brigade of Alabama, Georgia, and North Carolina troops. As Walker's men forced Seymour's back, aided by Lee's artillery fire, Ricketts's division entered the Cornfield, also to be torn up by artillery. Brig. Gen. Abram Duryée's brigade marched directly into volleys from Col. Marcellus Douglass's Georgia brigade. Enduring heavy fire from a range of 250 yards (230 m) and gaining no advantage because of a lack of reinforcements, Duryée ordered a withdrawal.^[13]

The reinforcements that Duryée had expected—brigades under Brig. Gen. George L. Hartsuff and Col. William A. Christian—had difficulties reaching the scene. Hartsuff was wounded by a shell, and Christian dismounted and fled to the rear in terror. When the men were rallied and advanced into the Cornfield, they met the same artillery and infantry fire as their predecessors. As the superior Union numbers began to tell, the Louisiana "Tiger" Brigade under Harry Hays entered the fray and forced the Union men back to the East Woods. The casualties received by the 12th Massachusetts Infantry, 67%, were the highest of any unit that day.^[15] The Tigers were beaten back eventually when the Federals brought up a battery of 3-inch ordnance rifles and rolled them directly into the Cornfield, point-blank fire that slaughtered the Tigers, who lost 323 of their 500 men.^[16]

While the Cornfield remained a bloody stalemate, Federal advances a few hundred yards to the west were more successful. Brig. Gen. John Gibbon's 4th Brigade of Doubleday's division (recently named the Iron Brigade) began advancing down and astride the turnpike, into the cornfield, and in the West Woods, pushing aside Jackson's men.^[17] They were halted by a charge of 1,150 men from Starke's brigade, leveling heavy fire from 30 yards (30 m) away. The Confederate brigade withdrew after being exposed to fierce return fire from the Iron Brigade, and Starke was mortally wounded. The Union advance on the Dunker Church resumed and cut a large gap in Jackson's defensive line, which teetered near collapse. Although the cost was steep, Hooker's corps was making steady progress.